| Congresso Brasileiro de Microbiologia 2023 | Resumo: 338-1 | ||||
Resumo:Under a One Health context, marine environments can play a pivotal role in spreading clinically significant antimicrobial resistance genes (ARGs) and acting as a source for pathogenic microorganisms. In this particular regard, members of the Vibrio genus are key inhabitants of marine and estuarine ecosystems widely known as active players in horizontal gene transfer in these environments, including clinically-important ARGs, and comprise different pathogenic species for humans and animals relevant for aquaculture and mariculture production. In this framework, the present study aimed to investigate the presence of beta-lactamase-encoding ARGs in Vibrio strains isolated from different marine ecosystems on the coast of Niterói city (RJ, Brazil). Over seven months, surface seawater samples (approximately 1,0 L, in triplicate) were collected from three distinct beaches, namely Icaraí, Jurujuba, and Piratininga, together with bivalve molluscs from the Perna perna species in a major mariculture farm. Bacterial isolation was performed on the selective media Thiosulfate Citrate Bile Salts Sucrose (TCBS) Agar and bacterial identification was pursued with MALDI-TOF MS. Genotypic detection of beta-lactam resistance was conducted using polymerase chain reaction (PCR), for the following genes: blaCTX-M-1,2, blaCTX-M-14, blaCTX-M-8, blaGES, blaSHV, blaTEM and blaKPC. A total of 280 colony-forming units (CFUs) were isolated on TCBS agar, with the majority of them from Jurujuba (n=97, 34.65%), followed by Piratininga (n=75, 26.78%), Icaraí (n=66, 23.57%) and the molluscs (n=42, 15%). Among the CFUs, a sum of 79 was identified as belonging to the Vibrio genus, including Vibrio alginolyticus (31.6%), Vibrio parahaemolyticus (34.2%), Vibrio navarrensis (7.6%), while the remaining 26.6% were classified as other species or not identified at the species level. In the genotypic screening, 44 Vibrio strains were analyzed until the present moment. The most prevalent beta-lactam resistance genes detected were blaSHV (n=15, 34.09%), followed by blaTEM (n=3, 6.82%) blaCTX-M-1,2 (n=2, 4.54%) and blaCTX-M-8 (n=2, 4.54%). The results of this study emphasize the significance of surveillance of antimicrobial-resistant strains in these estuarine ecosystems. Thus, Vibrio spp. might be exerting a crucial reservoir role in the maintenance of an ambient resistome that can represent risks to the public health of the local population through both direct contact with these recreative waters and ingestion of typical seafood from the area. Palavras-chave: Vibrionaceae, antibiotic resistance, marine ecosystems, One Health Agência de fomento:Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), processo: 306395/2020-7; Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro,processos: E26/200.948/2021 e E-26/211.284/2021 | |||||